| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.151829 |
| Chromosome: | chromosome_3 |
| Location: | 5077169 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre03.g181150 | DLL1,ROC106,FLA14,RSP22,LC8 | Dynein arm light chain 8, LC8 | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TTGCGGCCTACATCAAGAAGGAGTTCGACC |
| Internal bar code: | TCGGGGTAAGGGCTAGCACTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 875 |
| LEAP-Seq percent confirming: | 99.9446 |
| LEAP-Seq n confirming: | 1804 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATCTTCCTCCCCTTCCTTC |
| Suggested primer 2: | AAATGTATTTCGACCCTGCG |