| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.151834 |
| Chromosome: | chromosome 10 |
| Location: | 2373462 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g435200 | (1 of 4) IPR000533 - Tropomyosin | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGATTAGCACACACATGCCTGCTAGGATT |
| Internal bar code: | GCGGGAGACTCGGAACGCACCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 681 |
| LEAP-Seq percent confirming: | 98.3553 |
| LEAP-Seq n confirming: | 598 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGCCTTGTAACAAACGGTG |
| Suggested primer 2: | TGAGTTTGTGTTGCGAAAGC |