Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.151848 |
Chromosome: | chromosome_17 |
Location: | 3105982 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre17.g721250 | FAP22 | Flagellar Associated Protein, qilin-like | sense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | ATGAATACGGTCGAGTGGCGATTGGAAGAG |
Internal bar code: | CTGCGCGCTGGTGGTGACGTGCC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 929 |
LEAP-Seq percent confirming: | 99.7107 |
LEAP-Seq n confirming: | 1034 |
LEAP-Seq n nonconfirming: | 3 |
LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | AGGCCAACATGAACTTCGAC |
Suggested primer 2: | GCATTTGCGTCTGAAACTGA |