| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.151860 |
| Chromosome: | chromosome 1 |
| Location: | 7616093 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g054950 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTATTTCCGTTCGCCATCAGGCTTAAAACC |
| Internal bar code: | CAGCTACCTGGAGCAGCTAATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 856 |
| LEAP-Seq percent confirming: | 76.7163 |
| LEAP-Seq n confirming: | 1855 |
| LEAP-Seq n nonconfirming: | 563 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAAGTGAACCAGCCAATTT |
| Suggested primer 2: | ACCCCGCCTCTTTATCATTT |