| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.151862 |
| Chromosome: | chromosome 12 |
| Location: | 6878862 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g561000 | CYN28 | (1 of 1) PTHR11071//PTHR11071:SF286 - PEPTIDYL-PROLYL CIS-TRANS ISOMERASE // SUBFAMILY NOT NAMED; Cyclophilin 28 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGGGGTAGCCGCCAAGTCGACAAGGTAGC |
| Internal bar code: | GGTGGCCCGGTAAGGATTGATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 809 |
| LEAP-Seq percent confirming: | 99.726 |
| LEAP-Seq n confirming: | 3640 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAACAGCTGAAAGCCTGGAA |
| Suggested primer 2: | CGTGTGTAAATGCAGCGAGT |