| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.151864 |
| Chromosome: | chromosome 10 |
| Location: | 5231412 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g457100 | (1 of 1) 1.13.11.79 - 5,6-dimethylbenzimidazole synthase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAGTGGCGGCGGTGGCAGCAGGTATGGGC |
| Internal bar code: | TAGGATTTTTGCGCGCTCCCCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1170 |
| LEAP-Seq percent confirming: | 97.561 |
| LEAP-Seq n confirming: | 160 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCAGGAGCCCACTAACAGA |
| Suggested primer 2: | GTCAGCAGCCGTACAGAACA |