| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.151887 |
| Chromosome: | chromosome_17 |
| Location: | 4449238 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre17.g732150 | FAP130 | Flagellar Associated Protein | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | ACTTTCTTTCCTCCCTCCCGTCCTTCCCCA |
| Internal bar code: | CGGTTAGAGTTGTCGTAGGTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 905 |
| LEAP-Seq percent confirming: | 96.5928 |
| LEAP-Seq n confirming: | 567 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACATGAGTCGACCAGGGAC |
| Suggested primer 2: | CCGAAGTCTCGTCCTGTCTC |