| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.151898 |
| Chromosome: | chromosome 1 |
| Location: | 5193110 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g036150 | (1 of 1) PTHR14095:SF0 - PROTEIN C06G1.5, ISOFORM B | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTCTGTGGTGCAGGCACATATGCGTGAGC |
| Internal bar code: | TAGCGGGTCGGCAAGGCCCATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 945 |
| LEAP-Seq percent confirming: | 99.278 |
| LEAP-Seq n confirming: | 550 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGACCTATGGCACCAGTTG |
| Suggested primer 2: | ATGCAGCTCGTCAACACAAC |