Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.151944 |
Chromosome: | chromosome_12 |
Location: | 6143120 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre12.g536050 | ALA1 | Phospholipid-transporting ATPase | sense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GTCTCTCGACGACCGCGAGAGCAAGCTGGC |
Internal bar code: | ACCAGAGCAGCCGTAGTATCGG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 630 |
LEAP-Seq percent confirming: | 87.2263 |
LEAP-Seq n confirming: | 2151 |
LEAP-Seq n nonconfirming: | 315 |
LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CACGGATGAGAAGAGCATCA |
Suggested primer 2: | GCCTGAGCTCCAAGTCAATC |