| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.151946 |
| Chromosome: | chromosome 3 |
| Location: | 4132866 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g173400 | (1 of 5) PTHR10751 - GUANYLATE BINDING PROTEIN | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCCAGTGCGGCCTGCGGCGACTGCTTGGC |
| Internal bar code: | TACATAACTAGTTCCGACTAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 655 |
| LEAP-Seq percent confirming: | 99.7809 |
| LEAP-Seq n confirming: | 911 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TATTGGCGTTGACTGCTGAG |
| Suggested primer 2: | GCTGTGTGATACCGGTGTTG |