Insertion junction: LMJ.RY0402.151974_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.151974
Chromosome:chromosome 2
Location:3401704
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre02.g095106 PGP1 (1 of 2) 2.7.8.5 - CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase / Phosphatidylglycerophosphate synthase; Phosphatidylglycerolphosphate synthase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCCAAGTAATAAACGAGAGGCACGAGATTG
Internal bar code:GCACAATCGTCGTGCGCACCTC

Confirmation - LEAP-Seq

LEAP-Seq distance:410
LEAP-Seq percent confirming:99.711
LEAP-Seq n confirming:345
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:GAACACGGAGAAAGGGATGA
Suggested primer 2:CGAAGTGGTGAGAGGAGAGG