| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.152010 |
| Chromosome: | chromosome 3 |
| Location: | 5989016 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g190550 | (1 of 1) PF08638 - Mediator complex subunit MED14 (Med14) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGCGAGTGTGATTTGCACATATATCTGTA |
| Internal bar code: | GGGGATTCCTGAAGGCGGTGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 152 |
| LEAP-Seq percent confirming: | 98.8971 |
| LEAP-Seq n confirming: | 269 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTGTTGGTGAAGAGGGGTG |
| Suggested primer 2: | CTAGGCCTGCAAACGCTATC |