| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.152015 |
| Chromosome: | chromosome 1 |
| Location: | 4338133 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g029400 | (1 of 6) PTHR31942//PTHR31942:SF1 - FAMILY NOT NAMED // MLO-LIKE PROTEIN 1 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCCTGCAGGTGGGAGCCAGTGCGTGTGTG |
| Internal bar code: | CAGGCAAGCCAATAAGGTGCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 555 |
| LEAP-Seq percent confirming: | 92.8174 |
| LEAP-Seq n confirming: | 1667 |
| LEAP-Seq n nonconfirming: | 129 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAGCCTAGCACTATGCCTC |
| Suggested primer 2: | GAGTGAGGTGGAGTTGAGGG |