Insertion junction: LMJ.RY0402.152019_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.152019
Chromosome:chromosome 14
Location:3992363
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre14.g633300 (1 of 1) IPR003582//IPR006626//IPR009030 - ShKT domain // Parallel beta-helix repeat // Insulin-like growth factor binding protein, N-terminal intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTCAGCACCAAAACCACCAGTACAGGTTTC
Internal bar code:CAAGGATGTCCATGCCGCGCGA

Confirmation - LEAP-Seq

LEAP-Seq distance:128
LEAP-Seq percent confirming:99.4388
LEAP-Seq n confirming:886
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:ACGCACACACACACACACAC
Suggested primer 2:AGTAAGCACAAGGATCGCGT