| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.152049 |
| Chromosome: | chromosome 2 |
| Location: | 274452 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g074950 | (1 of 5) PTHR10351//PTHR10351:SF42 - TRANSCRIPTION FACTOR BTF3 // LD40663P | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTTCGACCCAGGGCATGTCATTACTGCGT |
| Internal bar code: | TGTCCATGATTCCACACCTCCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1123 |
| LEAP-Seq percent confirming: | 99.7844 |
| LEAP-Seq n confirming: | 1851 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCAGAACAAGGTAGGTGCCC |
| Suggested primer 2: | TGAGCTCGGTATTGCAAGTG |