| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.152060 |
| Chromosome: | chromosome_14 |
| Location: | 3760115 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre14.g632350 | FAP138 | Flagellar Associated Protein | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GTGAACACACCCGCGGCCGTCGCCACCGCC |
| Internal bar code: | GTTCGAATTTGCGCTTGCTTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 842 |
| LEAP-Seq percent confirming: | 98.8876 |
| LEAP-Seq n confirming: | 3378 |
| LEAP-Seq n nonconfirming: | 38 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACACGGTTTCACTCACTCCC |
| Suggested primer 2: | ACAAGATATTGGTGCAGCCC |