Insertion junction: LMJ.RY0402.152062_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.152062
Chromosome:chromosome 3
Location:3817161
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre03.g170550 POC4 Proteome of centriole protein 4 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGGCGCCTAGCGAGTCAGAACATAGTCAAC
Internal bar code:GTCTAGGCCCGACCTATGGTTA

Confirmation - LEAP-Seq

LEAP-Seq distance:779
LEAP-Seq percent confirming:99.3311
LEAP-Seq n confirming:594
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:CGCACTGACATGCAACTTCT
Suggested primer 2:TGCTCATGGTGTAGTCGCTC