| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.152063 |
| Chromosome: | chromosome 17 |
| Location: | 4416940 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g731750 | CYP740A1,CYP37 | Cytochrome P450, CYP26 superfamily; (1 of 1) 1.14.13.77 - Taxane 13-alpha-hydroxylase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGGCCCGGCAAGAAGAAACTTGCTCCCAA |
| Internal bar code: | AGCTGTCGGTGCGTCGGCCTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 690 |
| LEAP-Seq percent confirming: | 99.8603 |
| LEAP-Seq n confirming: | 2859 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGAGACACGATGAGAGGCAC |
| Suggested primer 2: | GTGCCTCTCATCGTGTCTCA |