| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.152067 |
| Chromosome: | chromosome 3 |
| Location: | 6449877 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g194700 | AGL1 | (1 of 1) 3.2.1.20//3.2.1.3 - Alpha-glucosidase / Maltase-glucoamylase // Glucan 1,4-alpha-glucosidase / Lysosomal alpha-glucosidase; Alpha glucosidase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACCGCCTGTCAGCCTGAGGCAGGCAGTAG |
| Internal bar code: | TGAGCTCGTTAGGGGAGATAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 429 |
| LEAP-Seq percent confirming: | 99.1846 |
| LEAP-Seq n confirming: | 1338 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCGTACATTCACCCACTGT |
| Suggested primer 2: | TCTATGTCGCTCCACAGCAC |