Insertion junction: LMJ.RY0402.152078_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.152078
Chromosome:chromosome 12
Location:7947723
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre12.g552300 CTL3 (1 of 1) IPR001304//IPR016187//IPR024616 - C-type lectin // C-type lectin fold // Pherophorin; Putative pherophorin with C-type lectin intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGCTGACGTGTGCATTTGCGCCACTGCGCG
Internal bar code:AAGAATGGGCGGTTGGGTGGAA

Confirmation - LEAP-Seq

LEAP-Seq distance:171
LEAP-Seq percent confirming:90.2439
LEAP-Seq n confirming:185
LEAP-Seq n nonconfirming:20
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:GCGGCTCCATCTACTACGAG
Suggested primer 2:CCAGCCCAAACTAAACCAAA