| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.152082 |
| Chromosome: | chromosome 3 |
| Location: | 2933755 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g163150 | (1 of 2) K09955 - hypothetical protein (K09955) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACACACGCCCTCGCTCACTCATGGCAGGC |
| Internal bar code: | TATGTTTTTATAGGCAGCCTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 48 |
| LEAP-Seq percent confirming: | 99.8262 |
| LEAP-Seq n confirming: | 2297 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATGTAGAGGATTTGGGCGT |
| Suggested primer 2: | CTCGTATTCACCAGAACGCA |