| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.152090 |
| Chromosome: | chromosome 12 |
| Location: | 2294657 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g507750 | (1 of 1) PF00069//PF07714//PF13637 - Protein kinase domain (Pkinase) // Protein tyrosine kinase (Pkinase_Tyr) // Ankyrin repeats (many copies) (Ank_4) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGGGGAGGAGAGGGGTGCTCTGGGGGCAG |
| Internal bar code: | CCCACTGTCCAGATCCGTAATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 709 |
| LEAP-Seq percent confirming: | 98.6308 |
| LEAP-Seq n confirming: | 2017 |
| LEAP-Seq n nonconfirming: | 28 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCTGTTTCGTCCACTGTCC |
| Suggested primer 2: | CCAAGGCCACTCAGAGCTAC |