Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.152092 |
Chromosome: | chromosome_17 |
Location: | 3439688 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre17.g724550 | DIP13 | Deflagellation inducible protein | sense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CATGCATACAACGCCTTGGGCACCGCGACT |
Internal bar code: | GCGCTCGTCGCGGAGGGCGTGG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 841 |
LEAP-Seq percent confirming: | 96.7708 |
LEAP-Seq n confirming: | 929 |
LEAP-Seq n nonconfirming: | 31 |
LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | AAGCGAGAGGCTGTGAACAT |
Suggested primer 2: | TGCTGTGAGTCTTGCATTCC |