| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.152095 |
| Chromosome: | chromosome 3 |
| Location: | 517421 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g145267 | (1 of 1) 3.5.4.3 - Guanine deaminase / Guanine aminase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTTGTTCCATCCGCTTGACTCCATCTGTG |
| Internal bar code: | CCACTTCCCCGGACTTGAGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1002 |
| LEAP-Seq percent confirming: | 93.787 |
| LEAP-Seq n confirming: | 951 |
| LEAP-Seq n nonconfirming: | 63 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCTAGAACCAGTCGAGTCC |
| Suggested primer 2: | AAGGCCAAGGTGAAGAAGGT |