| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.152107 |
| Chromosome: | chromosome 5 |
| Location: | 3007871 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g238374 | (1 of 1) K11975 - E3 ubiquitin-protein ligase RNF144 [EC:6.3.2.19] (RNF144) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTGGGACACAGCCAAACCGCTGCAGCAGC |
| Internal bar code: | ATGGGTCCGACGCCTCACTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 613 |
| LEAP-Seq percent confirming: | 87.1063 |
| LEAP-Seq n confirming: | 40237 |
| LEAP-Seq n nonconfirming: | 5956 |
| LEAP-Seq n unique pos: | 83 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGCAGAGCCACCCGTAGT |
| Suggested primer 2: | CATTTGTGACGTTGTGGGAG |