| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.152114 |
| Chromosome: | chromosome 6 |
| Location: | 2876341 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g272050 | FAP15,GPM1,PGM1 | (1 of 1) K15633 - 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (gpmI); Phosphoglycerate mutase 1 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACCTCTGTAGCCCCCGCTTACGCCTTGAC |
| Internal bar code: | TCAACAGGACAGTCGGACGGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1004 |
| LEAP-Seq percent confirming: | 95.2191 |
| LEAP-Seq n confirming: | 478 |
| LEAP-Seq n nonconfirming: | 24 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTGCAGCTGGACGTATCTG |
| Suggested primer 2: | TCTTGCAGTTGGTCCAGTGA |