| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.152146 |
| Chromosome: | chromosome 10 |
| Location: | 2195402 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g433800 | CSE21 | Predicted protein of CSE family | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGCCATGCGGCGGAGTAGGTGCCGCCGCA |
| Internal bar code: | AATCCCATCCTTCTTATGAAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 464 |
| LEAP-Seq percent confirming: | 99.465 |
| LEAP-Seq n confirming: | 2045 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTCCTCAACAGCACAGAGG |
| Suggested primer 2: | AGGTGCTTATCGTGGACCAG |