| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.152168 |
| Chromosome: | chromosome_1 |
| Location: | 4323680 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre01.g029250 | AAH1 | Aromatic amino acid hydroxylase-related protein | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TCGCTCACTGCTGCTTGTGACGGCCGCTGA |
| Internal bar code: | CGGATCCGGTACGCCGGATACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 182 |
| LEAP-Seq percent confirming: | 99.1477 |
| LEAP-Seq n confirming: | 349 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGTGGGTCCAGCTTTCTCT |
| Suggested primer 2: | GGACTCAGGGAGGGCTTATC |