Insertion junction: LMJ.RY0402.152188_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.152188
Chromosome:chromosome 6
Location:5655945
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre06.g286200 (1 of 4) PTHR22911//PTHR22911:SF6 - ACYL-MALONYL CONDENSING ENZYME-RELATED // SOLUTE CARRIER FAMILY 35 MEMBER G1 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGGCAGGTTGGAGCGCAGTTTCGTGATGAG
Internal bar code:GCTCACCAGGGCGCCAAACTTC

Confirmation - LEAP-Seq

LEAP-Seq distance:890
LEAP-Seq percent confirming:93.023
LEAP-Seq n confirming:7293
LEAP-Seq n nonconfirming:547
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:TACCAACCGCCATCACAGTA
Suggested primer 2:AGGGAACTGGCATGTGTTTC