| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.152212 |
| Chromosome: | chromosome 10 |
| Location: | 2820226 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g439450 | (1 of 7) PF05517 - p25-alpha (p25-alpha) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTCCCCGCCCACTCCCCCGGCACGGCCCT |
| Internal bar code: | GCCTATCGGCCCGCCAAGGCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 561 |
| LEAP-Seq percent confirming: | 72.0157 |
| LEAP-Seq n confirming: | 368 |
| LEAP-Seq n nonconfirming: | 143 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCAGGCACTAACATTCAAA |
| Suggested primer 2: | ATCTCCGGCTGTAAATGTCG |