| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.152220 |
| Chromosome: | chromosome_9 |
| Location: | 3444531 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre09.g389282 | FAP114 | Flagellar Associated Protein, C1a-32 | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GGGCGGCTCACGGGGTTTGGGTCTGCGCAA |
| Internal bar code: | TAAAGAATGGGTGTGGTGATCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 653 |
| LEAP-Seq percent confirming: | 34.4444 |
| LEAP-Seq n confirming: | 155 |
| LEAP-Seq n nonconfirming: | 295 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGCAGGGACTGGGTATAAG |
| Suggested primer 2: | TCTGCAAGTGTCCTGAGGTG |