Insertion junction: LMJ.RY0402.152223_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.152223
Chromosome:chromosome_9
Location:5020228
Confidence (%):95
Locus systematic id Locus common name Defline Orientation Feature
Cre09.g398104 antisense 3'UTR

Insertion site details

Flanking sequence (orientation from cassette outwards):ATCGTCCGGAGGGCGGGCCACGCACCAACG
Internal bar code:GAGGGTACGACTGTACGGGCGG

Confirmation - LEAP-Seq

LEAP-Seq distance:167
LEAP-Seq percent confirming:99.5113
LEAP-Seq n confirming:4276
LEAP-Seq n nonconfirming:21
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:CGCGGAAGGTAACAAGAGAG
Suggested primer 2:AGCGAGTGTGTGTGTGTCGT