| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.152243 |
| Chromosome: | chromosome 4 |
| Location: | 3288245 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g226850 | ASP1 | (1 of 1) 3.4.23.5 - Cathepsin D; Pepsin-type aspartyl protease | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCAGGGGGGCGGGAGCGTGGGAAAGCGCG |
| Internal bar code: | GAATTACCCGGTTGGCCAGTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 736 |
| LEAP-Seq percent confirming: | 98.802 |
| LEAP-Seq n confirming: | 3299 |
| LEAP-Seq n nonconfirming: | 40 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAACGACTCGTGGGTGTTGA |
| Suggested primer 2: | TAGCATCAAACTGCTCGGTG |