| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.152268 |
| Chromosome: | chromosome 7 |
| Location: | 2740947 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g331250 | (1 of 1) IPR008928//IPR012336 - Six-hairpin glycosidase-like // Thioredoxin-like fold | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCACGAGGTCACCATGGCGAGCTTGGTAAG |
| Internal bar code: | GACGGTGCAATTTGGCCATGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 258 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 458 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGCGTCAGGGAGGTAAGAG |
| Suggested primer 2: | TTCTTTCCACCATAGGCCAC |