| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.152279 |
| Chromosome: | chromosome 12 |
| Location: | 3404153 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g496900 | (1 of 1) IPR002711//IPR003615//IPR004919 - HNH endonuclease // HNH nuclease // Domain of unknown function DUF262 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGTCGGTTCCTATTGGATCCCAGGTGCGG |
| Internal bar code: | ACTGGGTGGGGTACGATGCCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 298 |
| LEAP-Seq percent confirming: | 55.5556 |
| LEAP-Seq n confirming: | 5 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCATGCTCTCGTAAACGTCG |
| Suggested primer 2: | AGGACCCAGGTGGAGAACTT |