Insertion junction: LMJ.RY0402.152280_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.152280
Chromosome:chromosome_7
Location:5019871
Confidence (%):58
Locus systematic id Locus common name Defline Orientation Feature
Cre07.g347050 RSP7 Radial spoke protein 7 sense 3'UTR

Insertion site details

Flanking sequence (orientation from cassette outwards):CTGTCGCCCAGTTCAAACTCGCCCAGCTGA
Internal bar code:ATCCGGGGTTTGAGTTCGGAGT

Confirmation - LEAP-Seq

LEAP-Seq distance:405
LEAP-Seq percent confirming:99.1886
LEAP-Seq n confirming:489
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:CTGGTCAACTTCATCTGCGA
Suggested primer 2:GTTGGTCCAAGACAGCAGGT