| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.152286 |
| Chromosome: | chromosome 3 |
| Location: | 2124789 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g157050 | (1 of 1) IPR020966 - Aluminum-activated malate transporter | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCCTTTAAATAGGAATCAGACCCCCCCCA |
| Internal bar code: | GCCTACTCACATTCCCGATTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 592 |
| LEAP-Seq percent confirming: | 98.5864 |
| LEAP-Seq n confirming: | 1604 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGCACCTATCCACTTTCAT |
| Suggested primer 2: | CTCAGGAAAGCGCTCCATAC |