| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.152303 |
| Chromosome: | chromosome_7 |
| Location: | 751373 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre07.g317850 | FBB10 | Flagellar/basal body protein | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TTGGAGCGGCAGATGGGCTATGGTGTGTAG |
| Internal bar code: | GCGAGTGAGCCCCTCCGTCTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 939 |
| LEAP-Seq percent confirming: | 97.5031 |
| LEAP-Seq n confirming: | 5467 |
| LEAP-Seq n nonconfirming: | 140 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAGTAAACTTGCGCATGAA |
| Suggested primer 2: | CATGTACGCATGGTTTCGTC |