| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.152310 |
| Chromosome: | chromosome 17 |
| Location: | 644524 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g700450 | GFY1 | (1 of 1) K07034 - uncharacterized protein (K07034); Putative acetate transporter | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAACCTAAACGGCGCGCTAGACGTAGAGGC |
| Internal bar code: | GCCGCAGACCACCACCGTTGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 485 |
| LEAP-Seq percent confirming: | 99.7627 |
| LEAP-Seq n confirming: | 5044 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCATCAGACAGCCGTTCTTG |
| Suggested primer 2: | CACGTGTCCGTTTGAATTTG |