| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.152351 |
| Chromosome: | chromosome 7 |
| Location: | 5729841 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g352800 | (1 of 1) PTHR35460:SF1 - TRANSLATION ELONGATION FACTOR EF-1 ALPHA | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCTGCGGCTCTGCATCGCAGTGTGGCGTT |
| Internal bar code: | CGATTCGCCGTAGGGTGCTCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 261 |
| LEAP-Seq percent confirming: | 58.9124 |
| LEAP-Seq n confirming: | 585 |
| LEAP-Seq n nonconfirming: | 408 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCACCCAGACTGAGGAGAAG |
| Suggested primer 2: | AATGCTTGTGGGTGATGTGA |