| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.152371 |
| Chromosome: | chromosome 5 |
| Location: | 410844 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g232900 | (1 of 4) PF02181 - Formin Homology 2 Domain (FH2) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAAATCAGAGCTCGCGCTCGACCCTTCGAG |
| Internal bar code: | AAGGTCAGATAGCCGCCGGGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 832 |
| LEAP-Seq percent confirming: | 99.8153 |
| LEAP-Seq n confirming: | 1621 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGATCCCAGGACGTTACAG |
| Suggested primer 2: | ACTTGACGCCACACACACAT |