| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.152424 |
| Chromosome: | chromosome_9 |
| Location: | 290370 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre09.g392867 | FMG1-B | Flagella membrane glycoprotein, major form | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | ACACGGCTAGCGGGACTCTGGATGCGGCGG |
| Internal bar code: | GAAAGGGCGGCACGAATTCATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 577 |
| LEAP-Seq percent confirming: | 99.236 |
| LEAP-Seq n confirming: | 1169 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACGTGGTGAATGTTGTTGC |
| Suggested primer 2: | ACCTACGGACAGATGGATCG |