Insertion junction: LMJ.RY0402.152425_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.152425
Chromosome:chromosome 3
Location:498360
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre03.g145187 (1 of 274) IPR020683 - Ankyrin repeat-containing domain intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGTGGGCTCGGCGCTCAAGAACAGGGCCAG
Internal bar code:ACGTGGCTCTCTCGCTGCGTGA

Confirmation - LEAP-Seq

LEAP-Seq distance:428
LEAP-Seq percent confirming:90.9513
LEAP-Seq n confirming:784
LEAP-Seq n nonconfirming:78
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:ACACACACACACACACACGG
Suggested primer 2:CGCACCACGTACACCACTAC