| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.152429 |
| Chromosome: | chromosome 12 |
| Location: | 8110055 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g551501 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAAATCTGTATGTAGCTTTTGTGTTCCGTC |
| Internal bar code: | GAACACCCCTCGTGGCGCCGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 417 |
| LEAP-Seq percent confirming: | 84.1315 |
| LEAP-Seq n confirming: | 896 |
| LEAP-Seq n nonconfirming: | 169 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGTTATGCCGTGCCCCTAC |
| Suggested primer 2: | AAAACTCAGCTTCCGTCGAA |