| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.152446 |
| Chromosome: | chromosome 1 |
| Location: | 5512385 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g038900 | (1 of 5) PTHR11614:SF87 - MONOGLYCERIDE LIPASE | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTTCAGAAATTGAGACGGCATGGCCGCGT |
| Internal bar code: | GAAAGGTTTTGGCTCATTCCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 180 |
| LEAP-Seq percent confirming: | 99.7173 |
| LEAP-Seq n confirming: | 2469 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCACCAGCTCCTCATCTTTC |
| Suggested primer 2: | GTACGGTTCCATCCCAAGTG |