| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.152457 |
| Chromosome: | chromosome 5 |
| Location: | 417913 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g232750 | CNK4 | NimA-related protein kinase; (1 of 1) PTHR24361//PTHR24361:SF311 - MITOGEN-ACTIVATED KINASE KINASE KINASE // SUBFAMILY NOT NAMED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TATAGCTTTAAGGGGCCGGCGGGACCGCCC |
| Internal bar code: | CCCATGGGCCAGCCTGCGAGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 661 |
| LEAP-Seq percent confirming: | 98.9195 |
| LEAP-Seq n confirming: | 4669 |
| LEAP-Seq n nonconfirming: | 51 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAGTTGGATGCTGCAGTTA |
| Suggested primer 2: | CTAGGGAAGACTCTGGTGCG |