Insertion junction: LMJ.RY0402.152459_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.152459
Chromosome:chromosome 13
Location:667090
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre13.g566200 CGL112 possible transcription factor; (1 of 19) PF00642 - Zinc finger C-x8-C-x5-C-x3-H type (and similar) (zf-CCCH) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTGCCAAACTCGTGGGTGATTGAAAGCCCA
Internal bar code:CGGGATTTAACAAGGGTCGACT

Confirmation - LEAP-Seq

LEAP-Seq distance:644
LEAP-Seq percent confirming:98.6432
LEAP-Seq n confirming:1963
LEAP-Seq n nonconfirming:27
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:CCCCTTATGTGCTGTGTGTG
Suggested primer 2:AAATGAACCAAACAGACGGC