| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.152467 |
| Chromosome: | chromosome_3 |
| Location: | 4781794 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre03.g178350 | FAP272 | Flagellar Associated Protein similar to calmodulin | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | ATGCACACAGCAACAAACAGTCCTTCACGT |
| Internal bar code: | GGCATCGGGCACCCGGTAGTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 126 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 26 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACAATGCCTCGGAAACAAC |
| Suggested primer 2: | AATGAGAAGGTCATGTGGGC |