Insertion junction: LMJ.RY0402.152519_3

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.152519
Chromosome:chromosome 9
Location:5278787
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre09.g399738 (1 of 239) IPR016024 - Armadillo-type fold intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGAACTCCGCCGGCGCCCTTGTTCATACAC
Internal bar code:GCATCTTAGCGGCAAACCGAAT

Confirmation - LEAP-Seq

LEAP-Seq distance:777
LEAP-Seq percent confirming:98.5916
LEAP-Seq n confirming:70
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:GAGGTGTACTGGCGAGGTGT
Suggested primer 2:TCTCCATCTAGGTCCATCCG