| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.152560 |
| Chromosome: | chromosome 4 |
| Location: | 2110856 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g219450 | DNJ30:MIDFRAG | (1 of 1) 1.3.1.21 - 7-dehydrocholesterol reductase / Sterol Delta(7)-reductase; Rieske [2Fe-2S] domain protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGCGTGGGCGACTCTGTAACATGCCCGGA |
| Internal bar code: | GAGGCATTCAAAGCGCTAGACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1093 |
| LEAP-Seq percent confirming: | 98.8337 |
| LEAP-Seq n confirming: | 1949 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TATGAATGCGACGGCATAAA |
| Suggested primer 2: | CAGTGGGTTAGAGACCCGAA |